M-UV96 MOUSE DRIVER DETAILS:
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M-UV96 MOUSE DRIVER
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There is increasing evidence for a role of SAgs in triggering these conditions In fact, studies from our laboratory have clearly established that MAM can trigger and cause flares in collagen-induced arthritis of mice 12and type 1 cytokines are known to be elevated in this model of m-uv96 mouse as well Our present finding that MAM also induces a similar type 1 cytokine response in mouse strains that are susceptible to mycoplasma-induced arthritis combined with our recent finding of antibodies to MAM in the sera from rheumatoid arthritis patients 55 further points to the value of the M. Although much of the work on the mechanisms of SAg action has been conducted with mice using the staphylococcal SAgs, particularly staphylococcal enterotoxin B SEBseveral findings presented here and elsewhere lead us to believe that the MAM SAg provides certain advantages for these studies.
First, M-uv96 mouse is produced by an organism that naturally infects rodents, resulting in a variety of syndromes, including arthritis.
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Also, MAM is much more potent for murine cells than m-uv96 mouse the staphylococcal SAgs, and on the basis of the present and previous data 4amounts of MAM which might be expected to be produced in the host by the organisms have been shown to profoundly affect immune functions in vivo. Our current studies are directed toward further defining the interaction of MAM with the murine immune system with a view to eventually understanding how SAgs determine polarization of the adaptive immune response. We thank William Sewell and Janice Weiss for their critical and useful comments on this manuscript. National Center for Biotechnology InformationU. Journal List Infect Immun v.
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Request an M-uv96 mouse Executive. Cytokines in test supernatants were quantitated in duplicate wells by a capture enzyme-linked immunosorbent assay ELISAusing a modification of the method of Schumacher et al. M-uv96 mouse, Mo. Optical density reading was performed at nm with a model UV well microtest plate spectrophotometer Bio-Rad, Hercules, Calif.
Cytokine concentrations are reported in picograms per milliliter. Gene-specific sequences were derived from GenBank submissions.
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The oligonucleotides used for these analyses have been published previously Sera were obtained from mice at m-uv96 mouse 7 after injection of MAM. Dilutions of serum were added to the wells, unbound sample was washed off, and the amount of bound murine immunoglobulin was detected by the addition of biotinylated IgG2a- or IgG1-specific antibodies, at concentrations of 0.
A simple linear regression analysis of the immunoglobulin titration-generated reference curve was used to extrapolate the amount of specific antibody contained in the test samples. These data are m-uv96 mouse in micrograms per milliliter. Mice were examined for severity of arthritis from 3 to 28 days postinjection.
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